curve auc values (ATCC)
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Curve Auc Values, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 878 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 878 article reviews
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1) Product Images from "Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle"
Article Title: Inhibition of Escherichia coli O157:H7 Growth Through Nutrient Competition by Non-O157 E. coli Isolated from Cattle
Journal: Microorganisms
doi: 10.3390/microorganisms13122811
Figure Legend Snippet: Bacteriocin presence and inhibitory activity of non-O157:H7 E. coli strains against O157:H7. ( A ) In vitro inhibition of E. coli O157:H7 by heat-inactivated cell-free supernatants of bovine non-O157:H7 strains under anaerobic conditions. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for each of the O157:H7 strains treated with heat-inactivated cell-free supernatant from each of the non-O157:H7 strains were calculated and compared to the mean AUC values for each O157:H7 strain grown in TSB alone to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean area under the curve (AUC) values from growth curves of E. coli O157:H7 indicator strains (ATCC 43888 and ATCC 700728) with and without exposure to heat-inactivated, cell-free supernatants of the bovine non-O157:H7 isolates. Four replicates were conducted for each inhibition assay. Statistical comparisons were made between both bovine non-O157:H7 isolate and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. ( B ) Antimicrobial peptides identified in bovine non-O157:H7 and E. coli O157:H7 isolates with Bagel5. Present genes are denoted in blue, and the absence of the gene is denoted in gray.
Techniques Used: Activity Assay, In Vitro, Inhibition
Figure Legend Snippet: Nutrient utilization profiles of non-O157:H7 E. coli strains as compared to two O157:H7 strains. ( A ) Percent difference in mean area under the curve (AUC) values for bovine non-O157:H7 strains compared to two E. coli O157:H7 strains (ATCC 43888 and ATCC 700728) in minimal media supplemented with different carbon sources. AUC values were determined from OD 630 growth curve data using GrowthCurveR (v0.3.1). Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dplyr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each strain. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD within the stats (v3.6.2) package in R. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. ( B ) Percent difference in mean AUC for bovine non-O157:H7 strains compared to ATCC 43888 and ATCC 700728 in minimal media supplemented with ethanolamine as either a carbon or nitrogen source. AUC values were determined from OD 630 growth curve data using GrowthCurveR. Mean AUC values for non-O157:H7 strains in each nutrient condition were calculated and compared to the mean AUC values for each O157:H7 strain to determine the percent difference (%diff) in AUC with dyplr (v1.1.4). Heatmap intensity corresponds to the %diff in the mean AUC values. Three replicates were conducted for each isolate. Statistical comparisons were made between bovine non-O157:H7 strains and each of the two E. coli O157:H7 strains with tukeyHSD using the stats (v3.6.2) package in R. ( C ) KEGG pathway completeness in bovine non-O157:H7 strains and both O157:H7 strains for the catabolism of ethanolamine, galactose, gluconate, glucuronate, mannose, and ribose. Blue denotes a gene as present and the intensity denotes the number of copies of that gene from the Eggnog-mapper (v2.18) annotation of that strain genome. Pathway completeness was determined with ggKegg (v1.1.18).
Techniques Used: